Fig. 5
The regional cytokines and protein regulation by TMF-treatment and AHRcKO at 48 h after MCAO. a The levels of the proinflammatory cytokines IL-1β, IL-6, and IFN-γ from the ipsilesional hemisphere were enhanced after stroke. The WT-TMF-treated and AHRcKO mice showed attenuated IL-1β, IL-6, and IFN-γ levels in the ipsilesional hemisphere after stroke. b–d Representative western blots of the peri-infarct cortex (b), the ipsilesional subventricular zone (SVZ) and striatum (c), and the ipsilesional hippocampus (d) from the normal WT, poststroke WT-vehicle, WT-TMF treated mice, the normal AHRcKO, poststroke AHRflx/flx, and AHRcKO mice (n = 6/each group), with β-actin as the loading control. The WT-vehicle and AHRflx/flx groups showed significantly unregulated protein levels of S100β and CXCL1 but downregulated levels in the WT-TMF group and AHRcKO mice in the peri-infarct cortex, the ipsilesional SVZ/striatum, and the ipsilesional hippocampus after MCAO. Furthermore, the protein level of NGN2 in the WT-TMF-treated and AHRcKO mice was upregulated when compared to the respective controls in the peri-infarct cortex, the ipsilesional SVZ/striatum, and the ipsilesional hippocampus after MCAO. +p < 0.05 AHRcKO-Normal compared with WT-Normal. #p < 0.05 compared with the respective normal controls. *p < 0.05 WT-TMF compared with the WT-vehicle and AHRcKO compared with the AHRflx/flx