Fig. 4

Association between chemerin enrichment and chemokine (C-C motif) receptor-like 2 (CCRL2) in 18.5-day-old fetal mice and 7-day-old offspring. a Protein levels of chemerin, CCRL2, and ChemR23 in whole brain tissues of 18.5-day-old fetal mice and 7-day-old offspring from controls and chemerin-induced diabetic mice (tissues from one whole brain). b Immunofluorescence staining for chemerin and CCRL2 in forebrain tissue specimens of offspring from the control, chemerin-induced diabetic dams, and chemerin-induced diabetic dams with CCRL2-knockdown mice. c Detection of chemerin and CCRL2 protein expression in brain tissue by western blotting (tissues from one whole brain). d The interaction between chemerin and CCRL2 was evaluated by co-immunoprecipitation and immunoblot assays in brain tissues of 18.5-day-old fetal mice (two fetal brains). Scale bar: 50 μm