Fig. 7

Role of chemerin in the activation of pyroptosis in vitro. a Protein levels of NLRP3, active caspase-3, active caspase-7, and active caspase-8 were detected by western blotting in macrophages treated with chemerin. Macrophages were isolated from the peritoneal cavity of normal mice and stimulated with 10 nM chemerin for 3 and 12 h, respectively. b Measurement of lactate dehydrogenase (LDH) activity in macrophages treated with chemerin for 3 and 12 h. c Protein expression of pro-caspase-1, pro-IL-1β, and pro-IL-18 in macrophages (left) and the levels of caspase-1, IL-1β, and IL-18 in the culture supernatants of macrophages (right) under the chemerin treatment condition. *Chemerin group vs. controls. #Chemerin group with ChemR23 knockdown vs. chemerin group, under the chemerin treatment for 3 h. @Chemerin group with ChemR23 knockdown vs. chemerin group, under chemerin treatment for 12 h. **, ##, and @@—P < 0.01