Fig. 4
From: Poldip2 mediates blood-brain barrier disruption in a model of sepsis-associated encephalopathy
Poldip2 and Cox2 co-localize in brain endothelial cells. Immunofluorescence microscopy was used to examine Poldip2 and Cox-2 localization in vivo. Poldip2+/+ mice were treated with LPS (18 mg/kg, IP) or PBS for 18 h before brains were isolated and prepared for staining. Antibodies against PECAM-1 (red), Cox-2 (green), and Poldip2 (pseudo-colored in blue) were used. Composite image depicts overlay of Cox-2, Poldip2, and PECAM-1 images. Scale bars equal 20 μm. Representative images are shown. n = 3–4 mice/group