Fig. 8
From: Poldip2 mediates blood-brain barrier disruption in a model of sepsis-associated encephalopathy
Poldip2 mediates LPS-induced BBB permeability via Cox-2. a Schematic of the experimental design. Evans blue dye was used to assess the effect of meloxicam on LPS-induced BBB permeability in Poldip2+/+ and Poldip2+/− mice. Animals received PBS or LPS (18 mg/kg, IP) for 18 h, meloxicam (5 mg/kg) subcutaneously at time 0 and 10 h later, or both LPS and meloxicam. Evans blue dye was administered by intravenous injection and allowed to circulate for 10 min before animals were sacrificed. b The graph depicts Evans blue dye concentration extracted from whole brains normalized to dry brain weight. Bars represent mean ± SEM. Two-way ANOVA, ****p < 0.0001 vs. Poldip2+/+ + PBS, ###p < 0.001 vs. Poldip2+/+ + LPS, ####p < 0.0001 vs. Poldip2+/+ + LPS, n = 3–7 animals/group