Fig. 2

Fas signaling pathway is required for death of RGCs and loss of axons in microbead-induced model of glaucoma. a IOP measurements were taken by rebound tonometry in WT and Fas-deficient Fas^lpr mice injected with microbeads or saline. Data is presented as mean IOP ± SD, N = 6 mice per group. IOP was significantly elevated on days 3–21 in WT and LPR mice that received microbeads as compared with WT control mice receiving saline (****P < 0.0001). b Representative confocal images of retinal flatmounts isolated at 28 days post-microbead or saline injection and stained with an anti-Brn3a antibody (red, RGC-specific marker) and a DAPI nuclear stain (blue) (scale bar, 50 μm). c Quantification of Brn3a positive RGCs represented as the mean RGC density/mm² retina ± SD. N = 6 per group, ***P<0.001, **P<0.01. d Representative photomicrographs of PPD-stained optic nerve cross-sections at 28 days post-microbead or saline injections (scale bar, 20 μm). e Quantification of healthy axons represented as the mean axon density (10⁴)/mm² ON ± SD. N = 6 per group, ****P < 0.0001