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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: A small peptide antagonist of the Fas receptor inhibits neuroinflammation and prevents axon degeneration and retinal ganglion cell death in an inducible mouse model of glaucoma

Fig. 2

Fas signaling pathway is required for death of RGCs and loss of axons in microbead-induced model of glaucoma. a IOP measurements were taken by rebound tonometry in WT and Fas-deficient Faslpr mice injected with microbeads or saline. Data is presented as mean IOP ± SD, N = 6 mice per group. IOP was significantly elevated on days 3–21 in WT and LPR mice that received microbeads as compared with WT control mice receiving saline (****P < 0.0001). b Representative confocal images of retinal flatmounts isolated at 28 days post-microbead or saline injection and stained with an anti-Brn3a antibody (red, RGC-specific marker) and a DAPI nuclear stain (blue) (scale bar, 50 μm). c Quantification of Brn3a positive RGCs represented as the mean RGC density/mm2 retina ± SD. N = 6 per group, ***P<0.001, **P<0.01. d Representative photomicrographs of PPD-stained optic nerve cross-sections at 28 days post-microbead or saline injections (scale bar, 20 μm). e Quantification of healthy axons represented as the mean axon density (104)/mm2 ON ± SD. N = 6 per group, ****P < 0.0001

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