Fig. 1
Chronic NTG administration increased microglial P2Y12R expression in the TNC. a Representative Western blot showing P2Y12R protein levels in the TNC following NTG injection. The band intensities are presented relative to those of β-actin. Data are presented as mean ± SEM; n = 6 per group; **p < 0.01 compared with the sham group. b Representative immunofluorescence images of P2Y12R in the TNC after NTG administration. The left graphs show the TNC regions, which marked by the white dotted line frames. The right graphs show higher magnification images of P2Y12R in the TNC. Scale bar: 20 μm. c, d Quantitative analysis of the number of P2Y12R-immunoreactive (ir) cells (c) and the P2Y12R immunoreactive area in the TNC (d) in a 320 × 320 μm2 visual field of each section per mouse after NTG injection. Data are presented as mean ± SEM; n = 4 per group; six sections from each mouse; *p < 0.05 and **p < 0.01 compared with the sham group. e, f Double-immunofluorescence labeling of P2Y12R with iba-1, NeuN, and GFAP in the TNC after NTG administration. The graphs in e show the TNC location which is marked by the white dotted line frame. The graphs in f show higher magnification images. Scale bar: 20 μm