Fig. 3
From: Cellular source of hypothalamic macrophage accumulation in diet-induced obesity
Bone marrow transplant study using LysMGFP donor and C57 recipient mice. a Images of whole mouse body irradiation with a head shield prior to BMT. b Representative FACS data confirming a successful BMT. The percentages indicate the proportion of LysMGFP cells among the peripheral blood monocytes. c Representative confocal images of GFP and DAPI immunostaining in the liver, skeletal muscle, choroid plexus, and hypothalamus of C57 recipients on a HFD for 12 and 30 weeks prior to sacrifice. Asterisks denote LysMGFP cells along the meningeal lining. Scale bars, 100 μm. ARC, hypothalamic arcuate nucleus; D3V, dorsal third ventricle; ME, median eminence; 3V, third ventricle