Fig. 2

LPS stimulated the expression of RagA in neurons. a After 24 h LPS treatment, brain tissues were prepared and analyzed by immunostaining with antibodies against RagA and NeuN while the cell nuclei were indicated by DAPI staining. The stained tissues were imaged on a Zeiss confocal 800 fluorescence microscope (Carl Zeiss, Jena, Germany). b Quantitative analysis of RagA levels. Fluorescence intensity of RagA signals in the forebrain cortex was quantified by a densitometric method and expressed in mean ± SD (n = 3). The difference between the experimental groups was analyzed by a two-tailed Student’s t test. **p < 0.01 (LPS vs control)