Fig. 3

LPS induced RagA expression and mTOR-p70S6K activation in the primary cortical neurons and neuronal stem cells. a Optimization of LPS concentration. After 24 h LPS treatment, primary rat cortical neurons were examined by staining with Hoechst 33342 and PI. PI-positive and Hoechst-stained cells were enumerated under a Zeiss fluorescence microscope (Carl Zeiss, Jena, Germany) and analyzed by one-way ANOVA followed by Tukey’s test (n = 3). *p < 0.05. b Activation of RagA and mTOR pathways in LPS-stimulated primary neurons. After 24 h LPS treatment, primary cortical neurons were lysed and analyzed by western blotting for RagA, mTOR, phospho-mTOR expression, while GAPDH was analyzed as control. Representative blot was shown. c Quantitative analysis of RagA and p-mTOR. Western blots in b were determined by a densitometric method. The signals (means ± SD) from three independent experiments were analyzed by one-way ANOVA, followed by post hoc Tukey’s test. *p < 0.05; **p < 0.01 (LPS vs control). d Activation of RagA and mTOR pathways in LPS-stimulated neuronal stem cells. After 24 h LPS treatment, neuronal stem cells were lysed and analyzed by western blotting for the expression of RagA, mTOR, phospho-mTOR, p70S6K, and phospho-p70S6K, while GAPDH was analyzed as control. Representative blot was shown. e Quantitative analysis of RagA, phospho-mTOR, and phospho-p70S6K. Western blots in d were determined by a densitometric method. The signals (means ± SD) from three independent experiments were analyzed by one-way ANOVA, followed by post hoc Tukey’s test. *p < 0.05; **p < 0.01; ***p < 0.001 (LPS vs control)