Skip to main content

Table 1 Published research examining the effects of probiotics intake on immune and inflammatory markers in animal and human models of MS

From: Immunomodulatory and anti-inflammatory effects of probiotics in multiple sclerosis: a systematic review

Author Sample Supplementation Duration Measurements Findings
Salehipour et al., 2017 [35] MOG-induced EAE in Female C57BL/6 mice, 8–10 weeks old, n = 8 per group Treatment (T) groups:
T1: L. plantarum
T2: B. animalis
T3: Both of probiotics.
109 CFU
22 days - CD4+, CD25+, Foxp3+, IL-10, IL-4, TGF-β, GATA3
- IFN-γ, IL-17, IL-6, T-bet, and ROR-γt
T1, T2, and T3 (particularly in T3):
- Mitigation of EAE in mice through motivating polarization of CD4+ T-cells toward T-reg by induction of anti-inflammatory cytokines and transcription factors and inhibition of pro-inflammatory cytokines, thereby suppressing autoreactive T-cells proliferation and leukocyte infiltration into CNS
Secher et al., 2017 [51] MOG-induced EAE in male C57/BL6Jmice, 8–12 weeks old, n = 30–40 per group T groups:
T1: E. coli Nissle
T2: archetypal.
108 CFU
30 days - Z0–1, Claudin-8, Reg3γ, Reg3β
- CD4+, IFN-γ, IL-17, and TNF-α
- Reduced the severity of EAE induced by immunization with the MOG35-55 peptides
- Decreased secretion of inflammatory cytokines and an increased production of the anti-inflammatory cytokine by autoreactive CD4 T-cells, in peripheral lymph nodes and CNS
- Increased numbers of MOG-specific CD4+ T-cells in the periphery contrasting with severely reduced numbers in the CNS
- Repaired intestinal permeability dysfunction
Kobayashi et al., 2012 [52] PLP-induced EAE in SJL/J and MOG-induced EAE in C57BL/6 Female mice, 7 weeks old, n = 15 per group - T group:
L. casei
0.6–1.2 × 109 CFU
50 days - IL-17 and IFN-γ, IL-10 protein levels and mRNA,
- CD4+, CD25+, CD8+ T-reg cells
- Non-significant neurological symptoms or histopathological changes of the spinal cord (SC) in either model
- Improve neurological symptoms in the PLP-induced EAE in SJL/J mouse
Unregulated IL-17 production by antigen-stimulated lymphocytes of draining lymph nodes on day 7
- Enhanced production of IL-10 and an increase in the percentage of CD4+CD25+ T-reg
- Strong expression of IL-17 mRNA on day 12, but this expression was not enhanced by LcS administration
Kwon et al., 2013
[53]
MOG-induced EAE in C57BL/6mice
6–8 weeks old, n = 10 per group
The IRT 5 probiotics contains 108 CFU/g of each strain:
L. casei,
L. acidophilus,
L. reuteni,
B. bifidum,
Streptococcus thermophiles.
(final 5 × 108 CFU)
30 days IL-4 (Th2) and IL-10 (Th2) and CD4+ T-cells, Foxp3+, IL-2
- CD3+, Gr1+ or/and CD11b+ monocyte, B220+ B cells
- Th1/Th17; IFN-γ, TNFα and IL-17 protein levels and mRNA, IL-1β
- Prophylactic and therapeutic effects
- A T-cell-mediated inflammatory autoimmune disease of the CNS
- Pretreatment of IRT5 probiotics expressively suppressed EAE development
- Delayed the disease onset of EAE
- Inhibited the pro-inflammatory Th1/Th17 polarization, while inducing IL-10+ producing or/and Foxp3+ T-reg, both in the peripheral immune system and at the site of inflammation
Lavasani et al., 2010 [54] MOG-induced EAE in Female C57BL/6mice, 8–10 weeks, n = 5 per group T1: L. paracasei, DSM 13434;
T2: L. plantarum, DSM 15312;
T3: L. plantarum, DSM 15313;
T4: L. paracasei, PCC, 101;
T5: L. delbrueckii, subsp. L. bulgaricus, DSM 20081.
T6: T1, T2, and T3.
109 CFU
25 days - Th2 type cytokines,
- IL-4, IL-10, TGF-β1
CD25, CD4+T-cells
IL-27, TNF-α, IFN-γ, IL-17
- T1, T2, and T3 reduced inflammation in CNS and autoreactive T-cell responses
- T1 and T2 induced CD4+CD25+Foxp3+ T-reg in MLNs and enhanced production of serum TGF- β1, while T3 increased serum IL-27 levels
- T6 suppressed the progression and reversed the clinical and histological signs of EAE
- The suppressive activity correlated with attenuation of pro-inflammatory Th1 and Th17 cytokines followed by IL-10 induction in MLNs, spleen and blood
Kouchaki et al., 2016 [50] 18 to 55 years, n = 30 per group probiotic capsule:
L. acidophilus
L. casei
B. bifidum
L. fermentum
2 × 109 CFU
12 weeks B cell- hs-CRP Decreased serum insulin, hs-CRP- improve B cell function concentration
Salami et al., 2019 20 to 60 years
n = 24 per group
Probiotic capsules:
B. infantis, B. lactis, L. reuteri, L. casei, L. plantarum and L. fermentum
2 × 109 CFU
16 weeks - IL-6, TNF-α, hs-CRP
- IL-10
- Reduction in some of inflammatory markers
- Increase in IL-10 and nitric oxide levels
  1. Abbreviations: CD cluster of differentiation, CFU colony-forming unit, CNS central nervous system, EAE experimental autoimmune encephalomyelitis, GATA3 GATA binding protein 3, GM-CSF granulocyte-macrophage colony-stimulating factor, hs-CRP high-sensitivity C-reactive protein, IL interleukin, IFN-γ interferon gamma, MLNs mesenteric lymph nodes, MOG myelin oligodendrocyte glycoprotein, PLP proteolipid protein. ROR-γt RAR-related orphan receptor gamma, Th T-helper cells, TNFα tumor necrosis factor alpha, TGF-β transforming growth factor beta; T-reg regulatory T-cell