Fig. 8

Immunohistochemical evaluation of acute (day 14) and chronic (day 90) fDTH-EAE rat brain lesions. a Representative anti-FR-β, anti-CD68, anti-MRC-1, and anti-iNOS staining. The low-power scale bar is 2 mm, and the high-power scale bar is 50 μm. b Quantification of immunohistochemistry data. The area of anti-iNOS positivity is significantly higher in the chronic phase than in the acute phase. The positive staining area was normalized to the lesion hemisphere area (positive area %). *P < 0.05. c The iNOS/MRC-1 ratio was higher in the chronic phase than in the acute phase, but the difference did not reach statistical significance (P > 0.05). Error bars denote standard deviation