Fig. 4

Committed neurons are susceptible to LACV-induced apoptosis. Representative confocal images of (a, f, and k) mock or (b–e, g–j, and l–o) 3 dpi LACV-infected COs immunohistochemically labeled with LACV (green), neuronal phenotyping antibodies (white), activated poly caspases (magenta), and nuclei (blue). Images are grouped by row using the neuronal phenotyping antibody with Sox2 being top, DCX middle, and βIII tubulin bottom. The three middle columns are images of single channels overlaid on nuclei from 3 dpi LACV-infected COs that are labeled accordingly. The far-right column (e, j, and o) is a combination of all four labels. The insets in e, j, and o are enlarged images of the highlighted yellow boxes in each panel. The corresponding yellow arrows in the associated individual label panels highlight the cell of interest shown in the inset. The images in the a, f, and k mock column are a combination of all four labels. All images were taken with a × 63 objective and are maximum intensity projects of 3 μm z-stacks taken with a 0.5 μm step. Scale bar in B = 20 μm and applies to all panels