Fig. 6

Flow cytometry analysis of neuronal cells from mock- and LACV-infected COs. Mock- (a–d) and LACV-infected (e–h) COs were non-enzymatically digested into a single cell suspension and analyzed via flow cytometry as described in the “Methods” section. Two representative examples are shown. Live cells were identified and interrogated for expression of activated poly-caspases (y-axis) and LACV expression (x-axis). a, e Active caspase and LACV staining from the whole live cell population. Gating within the whole live cell population for Sox2 (b, f), DCX (c, g) and βIII tubulin-positive cells allowed for examination of active poly-caspase and LACV staining within each neuronal population. Proportions of LACV-infected (i), activated poly-caspase (j), and LACV-infected/activated poly-caspase double-positive (k) neuronal cells within mock (closed circles) or infected (open squares) COs are shown. A two-way ANOVA with a Sidak’s multiple comparisons test was used to determine significance. **p < 0.001, ****p < 0.0001