Fig. 5

Astrocytic TRPV1 promoted neuronal excitement after OGD. Whole-cell patch clamps were used to detect spontaneously AP (a), induced AP (b), and RMP (c) of the neurons pre-treated with the astrocytes medium supernatant. Histogram showed spike frequency (d), the spike half-width of AP (e), the number of AP firing in 10 pA injection current (f), and the amplitudes of AP (g) of each groups. n = 3~5 per group. *P < 0.05,  **P < 0.01, ***P < 0.001, n = 3 per group, based on a Tukey’s test after one-way ANOVA. Data are shown as the mean ± SEM