Fig. 10

Decreased expressions of CD44 and PSGL-1 on Th1 effector cells following migration across HIBCPP cells. Expression of the receptor CD44 and PSGL-1 present in the Th1 cells was quantified, via flow cytometry measurement after 4 h of migration. The dot plots show representative expression of CD44 and PSGL-1 on Th1 in different conditions: a unstained Th 1 cells, b Th1 not in contact with HIBCPP cells in uninfected condition (=Th1 CT UI), c Th1 present in the upper compartment of the well in uninfected condition (=Th1 non migrated UI), d Th1 cells present in the bottom of the well in uninfected condition (= Th1migrated UI), e Th1 not in contact with HIBCPP cells in E-30-infected condition (=Th1 CT E-30), f Th1 present in the upper compartment of the well in uninfected condition (=Th1 non migrated UI), g Th1 cells present on the bottom of the well in E-30-infected condition (= Th1 migrated E-30). Filters (n = 36 each) were pooled for each experiment and each condition. Representative data of four independent experiments are shown