Fig. 2

E-30 infection of HIBCPP cells enhances migration of naive T cells. Migration of naive CD3⁺, CD4⁺, CD8⁺ T cells across HIBCPP cells was evaluated via immunofluorescence imaging in the following conditions: UI, UI + CXCL12, E-30 and E-30 + CXCL12. T cells were stained with cell tracker green Alexa 488. After 4 h of migration across HIBCPP cells immunofluorescence imaging (10 FOV per condition) was taken for CD3⁺ (a), CD4⁺ (c), and CD8⁺ (e) T cells present in the bottom well. b, d, f represent quantifications of T cells present in the bottom well after 4 h of migration for CD3⁺ CD4⁺ and CD8⁺ respectively. g Shows the quantification of naive T cells in E-30 + CXCL12 condition. The data are shown as mean ± SD of 6 independent experiments each performed in triplicate. Statistical significance was calculated using a Tukey–Kramer test. p values are displayed as follows: *p < 0.05 and ***p < 0.0001. p < 0.05 (*) was reached comparing UI to UI + CXCL12; p < 0.0001 (***) was reached comparing UI to E-30 + CXCL12 and comparing CD8⁺ with CD3⁺ and CD4⁺ after E-30 + CXCL12 stimulation; p < 0.0001 (###) was reached comparing UI + CXCL12 to E-30 + CXCL12, and p < 0.0001 (§§§) was reached comparing E-30 to E-30 + CXCL12. The white scale bar represents 100 μm