Fig. 5

Migration of T cells following E-30 has no impact on TEER of HIBCPP cells. Barrier integrity of HIBCPP cells was evaluated via measurement of the transepithelial resistance (TEER) at T = 0 h (white bars), T = 24 h (gray bars), and T = 28 h (black bars) for every condition uninfected control (UI), UI + CXCL12, E-30, E-30 + CXCL12 and for every cell type. a shows the result in the absence of T cells (= no T cells); b shows the results with CD3⁺ c with CD4⁺, d with CD8⁺, e with Th1 and f with CVB4-specific T cells. Data are shown as mean ± SD of 6 independent experiments each performed in triplicate. Statistical comparisons with p values are shown for T = 24 h and T = 28 h compared with the respective UI or UI and apical CXCL12 (=UI + CXCL12) at (T = 0 h). For statistical analysis, a Tukey–Kramer test was used; p values are displayed as follows: p < 0.0001 (***). *** p < 0.0001 was reached comparing the values to the respective control without E-30 infection