Fig. 6

Migration of T cells following E-30 has no impact on the paracellular dextran flux of HIBCPP cells The paracellular flux of the low molecular weight molecule dextran-TexasRed (MW 1000) was measured at the end of the 4-h migration period. Quantification via fluorescent measurement for no T cells (a), CD3⁺ (b), CD4⁺ (c), CD8⁺ (d), Th1 (e), and for CVB4-specific T cells (f) was performed for each condition (UI, UI + CXCL12, E-30, E-30 + CXCL12). The data are shown as mean ± SD of 6 independent experiments each performed in triplicate. p values were obtained comparing the respective conditions in the absence or presence prior to E-30 infection, namely: UI and UI + CXCL12 versus E-30 and E-30 + CXCL12. Statistical significance was calculated using a Tukey–Kramer test. p values are displayed as follows: *p < 0.05 and *** p < 0.0001. *p < 0.05 and *** p < 0.0001 were reached comparing the values to the respective control without E-30 infection