Distinct migratory pattern of naive and effector T cells through the blood–CSF barrier following Echovirus 30 infection

Table 1 Antibodies used for the flow cytometry analysis

	Color	Company	Isotype	Reference
Mix 1 antibodies
CXCR3	APC	BD	Mouse IgG1 κ	561732
CCR4	PE	BD	Mouse IgG1 κ	561110
CCR6	FITC	Biolegend	Mouse IgG2b κ	353411
CD44	BV510	BD	Mouse IgG2b κ	BD563029
PSGL-1	BV421	BD	Mouse IgG1 κ	BD743478
CD62L	PE-Cy7	BD	Mouse IgG1 κ	565535
Mix 2 antibodies
LFA-1	APC	BD	Mouse IgG1 κ	BD551060
CCR7	PE	Biolegend	Mouse IgG2a κ	FAB197P025
CCR5	FITC	BD	Mouse IgG2a κ	561747
Integrin β7	BV510	Biolegend	Mouse IgG1 κ	304314
CD29	BV421	BD	Mouse IgG1 κ	BD563514

For T cell characterization, flow cytometry was performed for migrated versus non-migrated T cells. The antibodies displayed within the table were used. The following antibodies were grouped as CXCR3-APC, CCR4-PE, CCR6-FITC, CD44-BV510, PSGL-1-BV421, CD62-L-PE-Cy7 for Mix 1 and LFA-1- β1-APC, CCR7-PE, CCR5-FITC, CD49d-PE-Cy7, CD29-BV510, and β7-BV421 for Mix2. For both mixes live/dead-APC-Cy7 was present

ISSN: 1742-2094