Fig. 6
Increased anti-inflammatory, but decreased inflammatory, microglia in VPS35CX3CR1 cortex after ischemic stroke. The ischemic stroke experiment was carried out as described in the legend of Fig. 1a. Three days after the stroke, mouse brain sections were subjected to co-immunostaining analyses using indicated antibodies (a–d), and the homogenates from indicated area were subjected to Western blot analysis (e–g). a, b Representative images.CD206 (an anti-inflammatory microglia-specific marker); iNOS (a marker for pro-inflammatory microglia); GFP (a reporter of CX3CR1+ microglia/macrophage). c, d Quantification analyses (mean ± SEM; n = 5/group; *P < 0.05). e–g Western blot analysis of homogenates of cortex from control and mutant mice using indicated antibodies (mean ± SEM; n = 3/group; *P < 0.05)