Fig. 1

Astrocytes exposed to OGD plus RO increased the permeability of the bEnd.3 monolayer. a GFAP (green) was expressed in more than 95% of cultured cells, and the nuclei were counterstained with DAPI (blue). Scale bar, 100 μm. b The TEER value of bEnd.3 monolayer cultured with different ACMs for 24 h was assessed. c The ability of sodium fluorescein (NaF) to cross the bEnd.3 monolayer cultured with different ACMs for 24 h was assessed (n = 7). N, cultured with normal 10% FBS DMEM; ACM-Medium, cultured with ACM from untreated astrocytes; ACM-OGD 2 h+RO, cultured with ACM from astrocytes exposed to OGD for 2 h plus RO for 24 h; ACM-OGD 4 h+RO, cultured with ACM from astrocytes treated with OGD for 4 h plus RO for 24 h. *P < 0.05 compared with the N group; ^#P < 0.05 compared with the ACM-Medium group; and ^$P < 0.05 compared with the ACM-OGD 2 h+RO group, ANOVA plus SNK test (b, c)