Fig. 5

Effect of Ex-4 on neurological deficit scores, infarct area, EB invasion, and ZO-1 expression after MCAO. a GLP-1R (red) was expressed on GFAP (green)-positive cells in the rat brain, and the nuclei were counterstained with DAPI (blue) Scale bar, 20 μm. b Neurologic deficit scores of the different groups at 24 h after MCAO (n = 5). c Representative TTC staining images of brain sections from the different groups. d Quantification of cerebral infarct area at 24 h after MCAO. e Representative images of coronal brain sections from rats perfused with EB from the different groups. f Quantitative analysis of EB intensity in different groups by spectrofluorometry (n = 5). g Representative images of immunofluorescence staining for GFAP (red) and ZO-1 (green) in ipsilateral brain tissues (n = 4–5) and the concentration of Ex4 was 150 μg/kg. Scale bar, 20 μm. *P < 0.05 and **P < 0.01, ANOVA plus SNK test (b, d, f)