Fig. 7

Clemastine inhibits expression of IL-1β and NLRP3 protein of microglial cells insulted by oxygen glucose deprivation (OGD) in vitro. Double immunofluorescence staining showing the distribution of Iba-1-labeled (a, d, g, j, m, and p, green) and IL-1β (b, e, and h, red) and NLRP3 (k, n, and q, red) immunoreactive AMCs in vitro at 1 h after OGD and OGD+clemastine and their corresponding controls. The colocalized expression of Iba-1 and IL-1β or NLRP3 in AMCs can be seen in c, f, i, or i, o, r, respectively. Bar graphs (s and t) depicting significant increase in the immunofluorescence intensity of IL-1β and NLRP3 expression, respectively, following OGD challenge when compared with matched controls, clemastine reversed these changes. N=3. *P < 0.05. Scale bars: a–r 100μm