Fig. 5

PEA increases 2-AG levels in either unstimulated or SP-stimulated RBL-2H3 cells. a–c AEA, 2-AG, and PEA levels were quantified, by LC-MS, after that RBL-2H3 cells were treated with PEA (10 μM) or stimulated with SP (10 μM) in the presence or absence of PEA (10 μM) for 15 min at 37 °C in a 5% CO₂ atmosphere. Each bar shows the mean ± SEM. *p < 0.05 and ***p < 0.001 compared with vehicle. °°p < 0.01 compared with SP