Fig. 3

Microglial depletion blunts proinflammatory gene induction to TLR agonists. OHSC slices at 4DIV were treated with CSF1R inhibitor PLX3397 for 10 days to deplete microglia, followed by treatment of different TLR agonists for 16 h. Immune gene induction was assessed by RT-PCR. Microglial depletion reduced induction of a IL-1β and b TNFα in response to LPS (TLR4), IMQ (TLR7), and PolyI:C (TLR3). Microglial depletion either had no effect or enhanced induction of c CX3CL1 and d IFNγ. *p < 0.05, **p < 0.01 vs Control, †p < 0.05 vs respective TLR agonist; n = 3 replicates/group with at least two independent experiments per condition