Fig. 7

Microglial repopulation increases inhibitory immune signals, neuronal synaptic and trophic genes. OHSCs were treated with PLX3397 for 10 days in vitro (DIV) to deplete microglia and followed by microglial repopulation in PLX3397-free medium for different time points. Gene expression was measured by RT-PCR. a Microglial repopulation was associated with an increased induction of immune inhibitory and M2-anti-inflammatory genes CD200R (1.4-fold), IL-4 (2.7-fold), IL-10 (4.4-fold), CX3CR1 (5.6-fold), CX3CL1 (3-fold), GR/glucocorticoid receptor (8-fold), and Arg-1 (16.6-fold). b Microglial repopulation caused increased expression of neuronal synaptic and trophic genes mGluR2 (2.4-fold), mGluR3 (2.9-fold), PSD-95 (1.9-fold), synaptophysin/Syp (2.2-fold), NGF (4.8-fold), and BDNF (2.7-fold). *p < 0.05 vs control matched for days in vitro