Table 1 Depletion of microglia reduces immune activation in response to TLR agonists. Brain slices were treated with vehicle or underwent microglial depletion with PLX3397 for 10 days, followed by treatment with a TLR agonist. Gene induction was assessed by RT-PCR at 16 h. Mean ± SEM

Control

100 ± 2

100 ± 3

102 ± 1

100 ± 2

98 ± 6

101 ± 16

103 ± 4

98 ± 4

+PLX3397

29 ± 3^**

21 ± 2**

118 ± 18

202 ± 10**

64 ± 8*

21 ± 0.4**

57 ± 8**

20 ± 3**

Pam3C (TLR2)

9165 ± 603*

27429 ± 2181*

140 ± 2*

74 ± 11*

86 ± 6

71 ± 2*

265 ± 14*

15 ± 1*

+PLX3397

746 ± 10^††

3128 ± 162^††

81 ± 6^††

104 ± 1

52 ± 1

18 ± 0.3^††

455 ± 11^††

3 ± 0.1^††

PolyI:C (TLR3)

1145 ± 67*

2534 ± 44*

132 ± 7*

48 ± 14*

52 ± 4*

39 ± 0.5*

118 ± 3

55±

+PLX3397

74 ± 1^††

275 ± 2^††

84 ± 1^††

183 ± 15^††

68 ± 3

7 ± 0.5^††

85 ± 5

8 ± 0.4^††

LPS (TLR4)

8104 ± 225*

22460 ± 2788*

399 ± 21*

93 ± 4

80 ± 2

39 ± 1*

286 ± 15*

8 ± 0.4*

+PLX3397

1056 ± 11^††

3667 ± 178^††

106 ± 7^††

316 ± 12^††

94 ± 12

9 ± 0.5^††

512 ± 14^††

1 ± 0.02^††

IMQ (TLR7)

1606 ± 17*

2485 ± 9*

188 ± 4*

130 ± 5

81 ± 2

42 ± 3*

94 ± 3

34 ± 1*

+PLX3397

180 ± 4^††

560 ± 6^††

264 ± 7^††

277 ± 4^††

141 ± 26^††

7 ± 0.3^††

135 ± 9^††

10 ± 1^††

D-SL03 (TLR9)

9639 ± 334*

7211 ± 399*

209 ± 19*

165 ± 26*

92 ± 4

12 ± 0.1*

249 ± 15*

23 ± 0.1*

+PLX3397

1234 ± 21^††

1151 ± 4^††

151 ± 16^††

287 ± 10^††

79 ± 4

1 ± 0.01^††

209 ± 9

5 ± 0.1^††