Fig. 5

MSR1 overexpression promoted the pro-inflammatory polarization of RAW264.7 cells after treatment with myelin debris in vitro. a IF staining of iNOS and CD206 in MSR1 vector (Vec) and overexpression (OE) RAW264.7 cells after treatment with myelin debris for 24 h. Scale bar = 10 μm. b, c Quantitative PCR of pro-inflammatory macrophage marker genes (iNOS, TNF-α, and IL-1β) and anti-inflammatory macrophage marker genes (CD206, YM1/2, and Arg1) in MSR1 Vec and OE RAW264.7 cells before or after treatment with myelin debris for 24 h (n = 3 per group, values are the mean ± SD, NS indicates no significance, *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA). d, e Flow cytometric analysis of MSR1 Vec and OE RAW264.7 cells after treatment with myelin debris for 24 h. The percentages of F4/80+ iNOS+ and F4/80+ CD206+ macrophages in the different groups were calculated (n = 3 per group, values are the mean ± SD, *p < 0.05, ***p < 0.001, one-way ANOVA)