Fig. 6

The effects of SAHA on TCI-induced mechanical allodynia and neuroinflammation in the spinal dorsal horn. a Experimental paradigms. b The effect of i.p. administration of SAHA on mechanical allodynia of TCI rats (n = 8). c Immunofluorescent staining of HDAC1 (green), HDAC2 (green), GFAP (red), and Iba-1 (red) in the spinal dorsal horn of the Sham + vehicle, the TCI + vehicle, the TCI + SAHA, and the Sham + SAHA group on POD 21. Scale bar = 100 μm. Representative bands (d) and quantitative analysis of HDAC1, HDAC2, GFAP, Iba-1, TNF-α, IL-1β, and IL-6 (e) in the spinal dorsal horn of Sham + vehicle, the TCI + vehicle, the TCI + SAHA, and the Sham + SAHA group. (n = 4). Analysis was based on the mean gray values and normalized to β-actin. Data are expressed as mean ± SEM. *p < 0.05, **p < 0.01 ***p < 0.001 versus the Sham + vehicle group, and ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 versus the TCI + vehicle group