Fig. 3
IIV treatment decreased Aβ deposition in APP/PS1 mice. a, b Representative microscopy images of the cortex and hippocampus of AD (a) and AD+IIV mice (b) staining for Aβ plaques (purple) and with Hoechst for nuclear staining (blue) (scale bar, 200 μm). c–f Quantification of the percentage of the surface area, numbers and total area of Aβ plaques was performed in five equidistant slices separated by 240 μm per animal (n = 9, mean ± SEM, Student’s t test, *P < 0.05, **P < 0.01). i–k Biochemical analysis of soluble and insoluble Aβ1-40 and Aβ1-42 extracted from the cerebral cortex with RIPA (i), SDS (j), and FA (k) from 8-month-old APP/PS1 mice by ELISA (n = 5, mean ± SEM, Student’s t test, *P < 0.05, **P < 0.01)