Table 1 Real-time PCR primers used for quantification of mRNA expression in this study
Primer name | Sequence (5′ → 3′) | |
|---|---|---|
m-RVG-lamp2b | Forward | CACCATTTGGATGCCCGAGA |
Reverse | CCGTTGGATGCTCTCTTCCC | |
Hdac1 | Forward | ACGGCATTGACGACGAATCCTATG |
Reverse | CTGAGCCACACTGTAAGACCACTG | |
Hdac2 | Forward | AGACTGGAATAGGACGGACGGATG |
Reverse | GTCATTTACCCAAGGGCTGGCTAC | |
Hdac3 | Forward | TGGAACAGGTGACATGTATGAA |
Reverse | GAAAAGGTGCTTGTAACTCTGG | |
Hdac8 | Forward | GATACTATTGCCGGAGATCCAA |
Reverse | GATAGCGTTTTCCCTAGGATGA | |
Rela | Forward | GCTACACAGGACCAGGAACAGTTC |
Reverse | CTTGCTCCAGGTCTCGCTTCTTC | |
BCL-2 | Forward | GATGACTTCTCTCGTCGCTAC |
Reverse | GAACTCAAAGAAGGCCACAATC | |
Caspase-3 | Forward | GAAACTCTTCATCATTCAGGCC |
Reverse | GCGAGTGAGAATGTGCATAAAT | |
IL-1β | Forward | GCAGAGCACAAGCCTGTCTTCC |
Reverse | ACCTGTCTTGGCCGAGGACTAAG | |
IL-6 | Forward | ACGTAGCTAGCTAGTCGGTATG |
Reverse | TCGTAGCTTGGCTAGTCGATCG | |
TNF-α | Forward | ATGTCTCAGCCTCTTCTCATTC |
Reverse | GCTTGTCACTCGAATTTTGAGA | |
β-actin | Forward | GCTGTCCCTGTATGCCTCTG |
Reverse | CGCTCGTTGCCAATAGTGATG | |
MiRNA-193b-3p | Forward | AACTGGCCCTCAAAGTCCCGCT |
MiRNA-486-3p | Forward | gCGGGGCAGCTCAGTACAGGAT |
MiRNA-369-3p | Forward | accggccgcggAATAATACATGGTTGATCTTTT |
MiRNA-410-3p | Forward | ccgcgggAATATAACACAGATGGCCTGT |
MiRNA-136-3p | Forward | ccgcggCATCATCGTCTCAAATGAGTCT |
MiRNA-195-5p | Forward | cgccggTAGCAGCACAGAAATATTGGC |
Cel-miR-39 | Forward | cgUCACCGGGUGUAAAUCAGCUUG |
Universal reverse primers | Reverse | CAGGTCCAGTTTTTTTTTTTTTTTCGT |