Fig. 4
From: Sustained hyperammonemia induces TNF-a IN Purkinje neurons by activating the TNFR1-NF-κB pathway
The expression of mTNF-a is increased in the white matter and Purkinje layer of the cerebellum after 4 weeks of hyperammonemia and is normalized by infliximab. Double fluorescence staining for TNF-a mRNA (green) and calbindin (red, a), Iba-1 (red, b), or GFAP (red, c) is shown. The content of TNF-a mRNA in Purkinje neurons (d) and white matter (e) was quantified. The values are the mean ± SEM of 3 rats per group. Values that were significantly different from those of the control rats are indicated by asterisks, values that were significantly different from those of the HA VH rats are indicated by a, and values that were significantly different from those infliximab-treated control rats are indicated by b. *p ≤ 0.05; ****p ≤ 0.0001; a p < 0.05; aaaa p ≤ 0.0001; bbbb p ≤ 0.0001. C VH = vehicle-treated control rats; C INFLIX = control rats treated with infliximab; HA VH = vehicle-treated hyperammonemic rats; HA INFLIX = hyperammonemic rats treated with infliximab