Fig. 3

Behavioral tests of memory and anxiety. Morris water maze (MWM). a–d Shows the latency of four groups during 5-days training test at 3, 6, 9, and 12 months old, respectively. e–h Shows the number of target platform crossings of four groups of mice in the probe test at 3, 6, 9, and 12 months old, respectively. Data are presented as mean ± S.E.M. Statistical significance was calculated by ANOVA between the sex-and age- matched P301S and WTs. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Open field test. All mice were transferred to the laboratory 1 h before testing. i Shows the trajectory of mice running during 10 min in the OFT box recorded by a tracking system. Red point in the figure represents the start position of mice, and the blue point indicates the end position. j–n Shows the distance traveled in the nine squares of the box (central area) at the age of 4, 7, and 11 months, respectively. k–o Shows the freezing time in the four corner squares of the box (corner area) at the age of 4, 7, and 11 months, respectively. Data are presented as mean ± S.E.M. and scatter. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Nest building test. Nest building test scored at the 16th and 24th hour just after equal amounts of cotton were placed in each cage and lights were turned off. p, q Show the scores at the 16th and 24th time point, respectively. Statistical analysis was performed between the sex-and age-matched P301S and WTs. **p < 0.01, ***p < 0.001, ****p < 0.0001