Fig. 7

Effects of AOPPs-MSA on pyroptosis in BV2 cells. a BV2 cells were administered with LPS (1 μg/mL), ATP (5 mM), and different concentrations of AOPPs-MSA. Western blotting analysis was performed to determine the expression of IL-18, pro IL-1β, and IL-1β. Data are representative of at least 3 independent experiments and the values are presented as mean ± SEM. *P < 0.05 versus control group. b Effect of different concentrations of AOPPs-MSA induced LDH release. c BV2 cells were double stained by PI (red) and DAPI (blue) and pictures were captured by using confocal microscopy. d PI positive cells were calculated in five random fields. Data are representative of at least 3 independent experiments and the values are presented as mean ± SEM. Scale bar, 100 μm. *P < 0.05 versus control group