Fig. 2

Persistent accumulation of microglia at the tumour site for 13 days following implantation. Microglia (green) and tumour cells (red) were visualised under anaesthesia using adapted multiphoton microscope at 5 (a), 9 (b), and 13 (c) days following implantation. Scale bar, 50 μm. Arrows indicate periphery of lesion where microglia have accumulated. Arrowheads indicate tumour cells within lesion, which are no longer visible after 9 days. d Number of DsRed-positive cells 5, 9 and 13 days post-implantation (n = 8 fields of view/time point). ***P < 0.001; Kruskal-Wallis with Dunn’s post hoc test