Fig. 3
Tumour cells are retained superficially but not deeper into the brain parenchyma. Confocal images at 20x (a) and 63x (b, c) of microglia (green), tumour cells (red) and nuclei (blue) in coronal sections obtained from Cx3cr1GFP/+ mice killed 22 days following implantation of MDA-MB-231-DsRed cells under the imaging window. Scale bars, 50 μm. Arrowheads indicate presence of viable DsRed-expressing tumour cells on outer surface of pia mater. Arrows indicate DsRed signal adjacent to, or within, GFP-expressing microglia deeper into brain parenchyma