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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Pellino1 regulates neuropathic pain as well as microglial activation through the regulation of MAPK/NF-κB signaling in the spinal cord

Fig. 4

Spinal Peli1 knockdown inhibits CCI-induced astrocyte activation on the ipsilateral side of the dorsal horn. a Timeline schematic of experimental paradigm. b Representative images showing inhibitory effects of Peli1 shRNA on activation of astrocytes (GFAP) at 7 days after CCI with a high magnification image (boxed, scale bar, 50 μm). c Data summary of GFAP intensity (n = 4). One-way ANOVA (F3,12 = 26.3). d qPCR showing decreased GFAP mRNA levels in the ipsilateral spinal cord of Peli1 shRNA-treated mice on day 7 after CCI (n = 4–5). One-way ANOVA (F3,15 = 31.3). e, f Representative images and quantitation of GFAP intensity showing inhibitory effect of Peli1 shRNA on astrocytic activation at 14 days after CCI (scale bar 50 μm, n = 4). One-way ANOVA (F3,12 = 36.2). Results are expressed as the Mean ± SEM. **p < 0.01, ***p < 0.001 compared with indicated group

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