Pellino1 regulates neuropathic pain as well as microglial activation through the regulation of MAPK/NF-κB signaling in the spinal cord

Table 1 Primer sequences used for real-time polymerase chain reaction analysis

Gene name	Forward primer sequence (5'-3')	Reverse primer sequence (5'-3')
Peli1	CCTTGTCCATGTAAGTTTCTC	CAGAGTTCAGAAGTCTGGAAC
Iba1	GGATTTGCAGGGAGGAAAAG	TGGGATCATCGAGGAATTG
CD11b	TCCGGTAGCATCAACAACAT	GGTGAAGTGAATCCGGAACT
GFAP	GCACTCAATACGAGGCAGTG	GGCGATAGTCGTTAGCTTCG
iNOS	GGCAGCCTGTGAGACCTTTG	CATTGGAAGTGAAGCGTTTCG
TNF-α	CATCTTCTCAAAATTCGAGTGACAA	CCAGCTGCTCCTCCACTTG
IL-6	CACAGAGGATACCACTCCCAACA	TCCACGATTTCCCAGAGAACA
IL-1β	TGAGGCCCAAGGCCACAGGT	CATCTTCTCAAAATTCGAGTGACAA
CD86	ACGATGGACCCCAGATGCACCA	GCGTCTCCACGGAAACAGCA
Arg1	TTAGGCCAAGGTGCTTGCTGCC	TACCATGGCCCTGAGGAGGTTC
CD206	TCAGCTATTGGACGCGAGGCA	TCCGGGTTGCAAGTTGCCGT
Ym1	CACTGAACGGGGCAGGTCCAAA	ACCCCTGCCTGTGTACTCACCT
IL-10	GGCAGAGAACCATGGCCCAGAA	AATCGATGACAGCGCCTCAGCC
GAPDH	UUCUCCGAACGUGUCACGUTT	UUCUCCGAACGUGUCACGUTT

Peli1 Pellino1, Iba1 ionized calcium binding adapator molecule 1, CD11b cluster of differentiation 11b, GFAP glial fibrillary acidic protein, iNOS inducible nitric oxide synthase, TNF-α tumor necrosis factor α, IL-6 interleukin 6, IL-1β interleukin 1β, CD86 cluster of differentiation 68, Arg1 arginase 1, CD206 cluster of differentiation 206, Ym1 chitinase-like protein 3, IL-10 interleukin 10, GAPDH glyceradehyde-3 phosphate dehydrogenase

ISSN: 1742-2094