Fig. 2

Increased perivascular astrocytes in rTg-DI rats. a–l Brain sections from 3-month-old wild-type (a–c) and rTg-DI (d–f) rats and 12-month wild-type (g–i) and rTg-DI (j–l) rats were labeled with Amylo-Glo to detect fibrillar amyloid (blue), rabbit polyclonal antibody to collagen IV to detect cerebral microvessels (red), and goat polyclonal antibody to GFAP to identify astrocytes (green). Scale bars = 50 μm. m, n Enlarged images of the highlighted regions of panels i and l, respectively. Scale bars = 10 μm. o Quantitation of astrocyte numbers from wild-type rats (black bars) and rTg-DI rats (gray bars) in different brain regions at 3 and 12 months of age. Data shown are mean ± SD of n = 5–6 rats per group. Compared to wild-type rats the astrocyte numbers were markedly elevated in rTg-DI rats and increased from 3 to 12 months of age in measured brain regions. ***P < 0.001