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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Pathological modeling of TBEV infection reveals differential innate immune responses in human neurons and astrocytes that correlate with their susceptibility to infection

Fig. 4

Impact of TBEV on human glial cells. HNPCs were differentiated for 13 days and infected with TBEV-Hypr at MOI 10−2. a Cells in non-infected (NI) and infected (TBEV) co-cultures were immunostained with an antibody directed against GFAP (astrocytes, red) at 7 dpi. Nuclei were counterstained with DAPI. Scale bars = 20 μm. b Manual enumeration of GFAP-positive cells using ImageJ software. Normalization was performed relative to non-infected GFAP-positive cells at 24 hpi. c Cells immunostained with OLIG2 antibody were enumerated automatically. Normalization was performed relative to non-infected OLIG2-positive cells at 24 hpi. The results are expressed as the mean ± SD and are representative of two (oligodendrocytes) and three (astrocytes) independent experiments performed in triplicate. Statistical analysis was performed using a two-tailed unpaired t test with Graphpad Prism V6.0.1. ns, non-significant (p > 0.05); *p < 0.05

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Journal of Neuroinflammation

ISSN: 1742-2094