Fig. 4

Dynamin activity mediates TLR4-dependent ERK 1/2 phosphorylation, c-fos and TNF mRNA accumulation and TNF secretion. WT BMMCs were pre-incubated with dynasore (80 μM) for 30 min before LPS addition (500 ng/mL). After stimulus, the cells were incubated at 37 °C for the indicated times and processed to obtain total protein or total RNA for RT-PCR. a ERK1/2 phosphorylation, bc-fos mRNA accumulation, c TNF mRNA synthesis, and d TNF secretion. Data are presented as the mean ± SD of 3–4 independent experiments. ^&&p < 0.01, ^&&&p < 0.001 vs. the value at time zero or in non-stimulated cells; #p < 0.05, ##p < 0.01, ###p < 0.001 vs. vehicle-treated cells, as determined by two-way ANOVA and post-hoc Tukey’s test