Fig. 7

In vivo effects of impaired TLR-4 signaling on R6/1 mast cells. a WT, R6/1, Wsh, Wsh reconstituted with WT BMMCs (Wsh Rec WT), and Wsh reconstituted with R6/1 BMMCs (Wsh Rec R6/1) mice were administered i.p. with either saline solution (white bars) or LPS (black bars) (1 mg/kg). One hour later, the mice were euthanized, and peritoneal washes were obtained. TNF was quantified by ELISA in peritoneal washes. Data shown in the graph were obtained from eight WT animals, six R6/1 mice, ten Wsh mice, 12 Wsh Rec WT mice, ten Wsh Rec R6/1 mice per each treatment. Data are presented as the mean ± SD. b Same experimental groups (6 animals each) mentioned in (a) were i.p. administered with a sub-lethal dose of LPS (80 mg/kg) and the survival was monitored for 40 h. Each point on the lines represent the accumulated death rate. ^##p < 0.01 vs. vehicle-treated animals as determined by two-way ANOVA and post hoc Tukey’s test