Fig. 2
Chemokine transcription was induced in the brain in response to systemic LPS challenges. Relative expression of a genes encoding CC chemokines and b genes encoding CXC and CX3C chemokines in the brains of mice challenged daily with LPS for 2, 5 and 7 days compared with vehicle-injected controls. Gene expression analysis was performed using TaqMan low-density arrays and normalised to Tbp. Fold change was calculated by comparing normalised expression of each gene to that of a calibrator from the vehicle-injected control group using the ΔΔCT method. Data show means ± 1SEM. Significance was calculated using two-way ANOVA: *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001; n = 5 per group