Fig. 3

Myeloid cells and lymphocytes were recruited to the brain following peripheral LPS exposure. Recruitment of CD45^hi leukocytes to the brain after daily injections of LPS or vehicle for 2, 5 and 7 days as assessed by flow cytometry. a Percentage of CD11b⁺Ly6G⁺Ly6C^int neutrophils as a proportion of all single, live, CD45⁺ cells in the brain. b Mean fluorescence intensity of F4/80 and Ly6G expression by neutrophils in the brains of LPS-challenged mice compared with neutrophils in the blood of vehicle and LPS-injected mice. c Percentage of CD11b⁺Ly6G⁻Ly6C⁺ monocytes, CD11b⁺F4/80⁺Ly6C⁻ macrophages and d CD4⁺ T cells, CD8⁺ T cells, NK1.1⁺TCRβ⁻ NK cells and NK1.1⁺TCRβ⁺ NKT cells as a proportion of all single, live, CD45⁺ cells in the brain. For gating strategy, see Supplementary Figure 4. Data show means ± 1SD. Significance was calculated using two-way ANOVA: *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001; n = 3 (vehicle), n = 5 (LPS). Data are representative of two independent experiments