Fig. 7
Effect of BM and adjuvant therapy with vitamin B12 on Ccl3 gene methylation in the hippocampus. Percentage of methylated DNA in a region covering part of the promotor of Ccl3 gene. The absolute standard curve method was used to quantify the DNA amount, and data were expressed as a percentage of methylated DNA [(captured DNA/input DNA) × 100]. The effects of BM and vitamin B12 were compared using a 2-way ANOVA (Bonferroni post hoc test). P values in tables below the graphs refer to the 2-way ANOVA and asterisks refer to the post hoc test. *P < 0.05; **P < 0.01; ***P < 0.001