Fig. 7
Overexpression of REST diminished antinociceptive effect of miR-9-5p modified BMSCs on the sarcoma-inoculated mice. a The expression efficacy of LV-REST was confirmed by increased REST mRNA level. b Overexpression of REST reduced MOR mRNA level at 21 day post operation. c–e Overexpression of REST diminished miR-9-5p modified BMSCs induced increase of TNF-α (c), IL-6 (d), and IL-1β (e) in ipsilateral dorsal root ganglion at day 21 post operation. In each group, n = 6. f, g Overexpression of REST diminished miR-9-5p modified BMSCs induced alleviation of spontaneous flinches (f) and paw withdrawal threshold (g) at 3, 7, 14, and 21 days after sarcoma cell inoculation. The proximal part of the catheter was sutured into the subcutaneous tissue to secure the catheter from being removed. Mice were grouped into four categories: control group, sham group; bone cancer pain group, and bone cancer pain + BMSC group which received 6 × 106 cells/10 μL BMSC infusion every day started 2 days before murine sarcoma cell implantation till day 21 post operation. BMSC+miR-9 and LV-REST were delivered in parallel. Overexpression of REST diminished miR-9-5p modified BMSCs. In each group, n = 6. Mean ± SD is used to present all data. *p < 0.05, **p < 0.01, and ***p < 0.001