Fig. 6

The effects of repeated applications of LA on the expression of GFAP in the ipsilateral SDH on POD 6. a, b Western blot analysis showing that the level of GFAP was not significantly influenced by either nerve injury or LA treatment. a Typical blotting trace. b Summary of the blotting results. n = 5. c–g Typical immunohistochemical staining showing the distribution of GFAP IR in the SDH in five groups. c Sham + vehicle group; d sham + LA (30 μM) group; e SNI + vehicle group; f SNI + LA (10 μM) group; g SNI + LA (30 μM) group. GFAP positive astrocytes were much densely distributed in the superficial dorsal horn in the sham groups (c, d). Both the density and morphology of the cells were not obviously affected by the nerve injury or LA treatment (e–g). Scale bar, 100 μm. h Summary of gray value for GFAP staining in five groups. Relative gray value was calculated the same as that of Iba-1. n = 3