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Table 1 Antibodies used for immunostainings

From: Interleukin-9 regulates macrophage activation in the progressive multiple sclerosis brain

Primary antibodyFluorophoreOriginTargetDilutionSourceApplication
CX3CR1FITCRat (mAB; IgG2b)Chemokine C-X3-C receptor 11:160Biolegend, California, USAFC
CD206PEMouse (mAB; IgG1)Mannose receptor1:50Beckman Coulter, California, USAFC
HLA-DP, DQ, DRBV785Mouse (mAB; IgG2a)MHC class II antigen1:100BD BioscienceFC
CD11bPerCP-Cy5.5Mouse (mAB; IgG1)Alpha M integrin1:80Biolegend, California, USAFC
CD45PE-Cy7Mouse (mAB; IgG)Leukocyte common antigen1:100Beckman Coulter, California, USAFC
CD68APCMouse (mAB; IgG2b)CD68 110-kDa transmembrane glycoprotein1:20Miltenyi, GermanyFC
CD14APC-eFluor780Mouse (mAB; IgG1)Leucine-rich repeat (LRR) protein1:100eBioscienceFC
CD163VioBlueMouse (mAB; IgG1)Hemoglobin-haptoglobin scavenger receptor1:100Miltenyi, GermanyFC
IL-9RBV421Mouse (mAB; IgG2b,k)Interleukin-9 receptor alpha1:30BD BioscienceFC
IL-9RPEMouse (mAB; IgG1)Interleukin-9 receptor alpha1:20R&D Systems, Minnesota, USAFC
IgG1 IsotypePEMouse1:20R&D Systems, Minnesota, USAFC
IgG2b,k IsotypeBV421Mouse1:30BD BioscienceFC
MOGRabbit (pAB; IgG)Myelin oligodendrocyte glycoprotein1:50Proteintech, Manchester, UKIHC
HLA-DP, DQ, DRMouse (mAB; IgG1)MHC class II antigen1:50Dako, Glostrup, DenmarkIHC
CD68Mouse (mAB; IgG1)CD68 110-kDa transmembrane glycoprotein1:25Dako, Glostrup, DenmarkIHC
TMEM 119Rabbit (pAB; IgG)Transmembrane protein 1191:50Atlas Antibodies, Bromma, SwedenIHC
IL-9RabbitInterleukin-91:50Santa Cruz Biotechnology, INC.IHC/IF
IL-9RMouse (mAB; IgG)Interleukin-9 receptor1:50MAB290; R&D SystemsIHC
CD4Mouse (mAB; IgG1)CD4+ T lymphocytes1:50ThermoFisher, Rockford, USAIF
CD8Mouse (mAB; IgG1)CD8+ T lymphocytes1:50Dako, Glostrup, DenmarkIF
  1. List of antibodies used for immunohistochemistry, immunofluorescence, and flow cytometry