Table 1 Antibodies used for immunostainings
From: Interleukin-9 regulates macrophage activation in the progressive multiple sclerosis brain
Primary antibody | Fluorophore | Origin | Target | Dilution | Source | Application |
|---|---|---|---|---|---|---|
CX3CR1 | FITC | Rat (mAB; IgG2b) | Chemokine C-X3-C receptor 1 | 1:160 | Biolegend, California, USA | FC |
CD206 | PE | Mouse (mAB; IgG1) | Mannose receptor | 1:50 | Beckman Coulter, California, USA | FC |
HLA-DP, DQ, DR | BV785 | Mouse (mAB; IgG2a) | MHC class II antigen | 1:100 | BD Bioscience | FC |
CD11b | PerCP-Cy5.5 | Mouse (mAB; IgG1) | Alpha M integrin | 1:80 | Biolegend, California, USA | FC |
CD45 | PE-Cy7 | Mouse (mAB; IgG) | Leukocyte common antigen | 1:100 | Beckman Coulter, California, USA | FC |
CD68 | APC | Mouse (mAB; IgG2b) | CD68 110-kDa transmembrane glycoprotein | 1:20 | Miltenyi, Germany | FC |
CD14 | APC-eFluor780 | Mouse (mAB; IgG1) | Leucine-rich repeat (LRR) protein | 1:100 | eBioscience | FC |
CD163 | VioBlue | Mouse (mAB; IgG1) | Hemoglobin-haptoglobin scavenger receptor | 1:100 | Miltenyi, Germany | FC |
IL-9R | BV421 | Mouse (mAB; IgG2b,k) | Interleukin-9 receptor alpha | 1:30 | BD Bioscience | FC |
IL-9R | PE | Mouse (mAB; IgG1) | Interleukin-9 receptor alpha | 1:20 | R&D Systems, Minnesota, USA | FC |
IgG1 Isotype | PE | Mouse | – | 1:20 | R&D Systems, Minnesota, USA | FC |
IgG2b,k Isotype | BV421 | Mouse | – | 1:30 | BD Bioscience | FC |
MOG | – | Rabbit (pAB; IgG) | Myelin oligodendrocyte glycoprotein | 1:50 | Proteintech, Manchester, UK | IHC |
HLA-DP, DQ, DR | – | Mouse (mAB; IgG1) | MHC class II antigen | 1:50 | Dako, Glostrup, Denmark | IHC |
CD68 | – | Mouse (mAB; IgG1) | CD68 110-kDa transmembrane glycoprotein | 1:25 | Dako, Glostrup, Denmark | IHC |
TMEM 119 | – | Rabbit (pAB; IgG) | Transmembrane protein 119 | 1:50 | Atlas Antibodies, Bromma, Sweden | IHC |
IL-9 | – | Rabbit | Interleukin-9 | 1:50 | Santa Cruz Biotechnology, INC. | IHC/IF |
IL-9R | – | Mouse (mAB; IgG) | Interleukin-9 receptor | 1:50 | MAB290; R&D Systems | IHC |
CD4 | – | Mouse (mAB; IgG1) | CD4+ T lymphocytes | 1:50 | ThermoFisher, Rockford, USA | IF |
CD8 | – | Mouse (mAB; IgG1) | CD8+ T lymphocytes | 1:50 | Dako, Glostrup, Denmark | IF |