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Table 1 Antibodies used for immunostainings

From: Interleukin-9 regulates macrophage activation in the progressive multiple sclerosis brain

Primary antibody Fluorophore Origin Target Dilution Source Application
CX3CR1 FITC Rat (mAB; IgG2b) Chemokine C-X3-C receptor 1 1:160 Biolegend, California, USA FC
CD206 PE Mouse (mAB; IgG1) Mannose receptor 1:50 Beckman Coulter, California, USA FC
HLA-DP, DQ, DR BV785 Mouse (mAB; IgG2a) MHC class II antigen 1:100 BD Bioscience FC
CD11b PerCP-Cy5.5 Mouse (mAB; IgG1) Alpha M integrin 1:80 Biolegend, California, USA FC
CD45 PE-Cy7 Mouse (mAB; IgG) Leukocyte common antigen 1:100 Beckman Coulter, California, USA FC
CD68 APC Mouse (mAB; IgG2b) CD68 110-kDa transmembrane glycoprotein 1:20 Miltenyi, Germany FC
CD14 APC-eFluor780 Mouse (mAB; IgG1) Leucine-rich repeat (LRR) protein 1:100 eBioscience FC
CD163 VioBlue Mouse (mAB; IgG1) Hemoglobin-haptoglobin scavenger receptor 1:100 Miltenyi, Germany FC
IL-9R BV421 Mouse (mAB; IgG2b,k) Interleukin-9 receptor alpha 1:30 BD Bioscience FC
IL-9R PE Mouse (mAB; IgG1) Interleukin-9 receptor alpha 1:20 R&D Systems, Minnesota, USA FC
IgG1 Isotype PE Mouse 1:20 R&D Systems, Minnesota, USA FC
IgG2b,k Isotype BV421 Mouse 1:30 BD Bioscience FC
MOG Rabbit (pAB; IgG) Myelin oligodendrocyte glycoprotein 1:50 Proteintech, Manchester, UK IHC
HLA-DP, DQ, DR Mouse (mAB; IgG1) MHC class II antigen 1:50 Dako, Glostrup, Denmark IHC
CD68 Mouse (mAB; IgG1) CD68 110-kDa transmembrane glycoprotein 1:25 Dako, Glostrup, Denmark IHC
TMEM 119 Rabbit (pAB; IgG) Transmembrane protein 119 1:50 Atlas Antibodies, Bromma, Sweden IHC
IL-9 Rabbit Interleukin-9 1:50 Santa Cruz Biotechnology, INC. IHC/IF
IL-9R Mouse (mAB; IgG) Interleukin-9 receptor 1:50 MAB290; R&D Systems IHC
CD4 Mouse (mAB; IgG1) CD4+ T lymphocytes 1:50 ThermoFisher, Rockford, USA IF
CD8 Mouse (mAB; IgG1) CD8+ T lymphocytes 1:50 Dako, Glostrup, Denmark IF
  1. List of antibodies used for immunohistochemistry, immunofluorescence, and flow cytometry