Fig. 3

IHC of CD4+ T cells (green) and CD31-labeled endothelial layer of blood vessels (red) penetrating the FMNuc. a Composite image of control FMNuc at 14 dpo showing no infiltrating T cells. b Composite image of axotomized FMNuc at 14 dpo. c, d Magnified fields from boxes in b showing CD4+ T cells are not contained within CD31-labeled blood vessels. Note, CD4+ T cells marked with arrowheads in close proximity to FMN labeled with fluorescent Nissl stain (blue). Scale bar in b = 100 μm and applies to a and b. Scale bar in d = 50 μm and applies to c and d